Description
This handbook is a comprehensive guide for molecular biologists and researchers, offering detailed protocols and methodologies in molecular genetics and genomics. It covers key techniques such as DNA and RNA extraction, PCR, cDNA synthesis, and expression cloning. Advanced analytical tools like electrophoresis, blotting, and ELISA are discussed, along with modern technologies such as microarray, next-generation sequencing (NGS), and transcriptomics. The book also delves into genetic markers and polymorphic analyses using SSRs, RAPD, RFLP, AFLP, and innovative approaches like SAMPL. Designed as a practical resource, it is invaluable for gene expression studies, protein detection, and genetic analysis.
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